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Image Search Results
Journal: Frontiers in Cell and Developmental Biology
Article Title: Enhancing autophagy and energy metabolism in the meniscus can delay the occurrence of PTOA in ACLT rat
doi: 10.3389/fcell.2022.971736
Figure Lengend Snippet: Histological and immunofluorescence staining of intra-articular injection of rapamycin (A) . The result of HE staining (Scale bar: the left panel was 200 um, the right panel was 25 um) (B) . The result of Toluidine blue staining (Scale bar: the left panel was 200 um, the right panel was 25 um) (C) . The immunofluorescence results of Col2 (Scale bar: the left panel was 200 um, the right panel was 25 um) (D) . The immunofluorescence results of MMP13 (Scale bar: the left panel was 200um, the right panel was 25 um) (E) . HC/CC ratio under HE staining (F) . HC/CC ratio under toluidine blue staining (G) . The statistics of Col2 -positive cells (H) . The statistics of MMP13-positive cells. (I) . OARSI score in each group.
Article Snippet: HE staining solution, Toluidine blue staining powder and safranin O/fast green staining powder was buy from Sigma (United States), SDS-PAGE gel rapid preparation kit was attained from Beyotime Biotechnology (Shanghai)Co., Ltd. rapamycin was acquired from Shanghai yuanye Bio-Technology Co., Ltd., IL-1β was purchased from R&D Systems (Bio-Techne), 3-methyladenine (3-MA, autophagy inhibitor) was produced from TargetMol (United States),
Techniques: Immunofluorescence, Staining, Injection
Journal: Frontiers in Cell and Developmental Biology
Article Title: Enhancing autophagy and energy metabolism in the meniscus can delay the occurrence of PTOA in ACLT rat
doi: 10.3389/fcell.2022.971736
Figure Lengend Snippet: The protective effect of rapamycin on the meniscus extracellular matrix. (A) The expression of MMP13, Col1, and Col2 in Western Blot (B) . The gray value statistics of MMP13 (C) . The gray value statistics of Col1 (D) . The gray value statistics of Col2 (E) . The result of safranin O/fast green staining (Scale bar: the left panel was 200 um, the right panel was 25 um) (F) . The immunofluorescence staining results of MMP13 (Scale bar: the left panel was 200 um, the right panel was 25 um) (G) . The statistics of MMP13-positive cells.
Article Snippet: HE staining solution, Toluidine blue staining powder and safranin O/fast green staining powder was buy from Sigma (United States), SDS-PAGE gel rapid preparation kit was attained from Beyotime Biotechnology (Shanghai)Co., Ltd. rapamycin was acquired from Shanghai yuanye Bio-Technology Co., Ltd., IL-1β was purchased from R&D Systems (Bio-Techne), 3-methyladenine (3-MA, autophagy inhibitor) was produced from TargetMol (United States),
Techniques: Expressing, Western Blot, Staining, Immunofluorescence
Journal: Frontiers in Cell and Developmental Biology
Article Title: Enhancing autophagy and energy metabolism in the meniscus can delay the occurrence of PTOA in ACLT rat
doi: 10.3389/fcell.2022.971736
Figure Lengend Snippet: Influence of the meniscus on articular cartilage during the development of PTOA (A) . The immunofluorescence staining results of MMP13 in 1 week, 2 and 3 weeks after ACLT surgery (Scale bar: the left panel was 100 um, the middle and the right panel was 25 um). (B) The statistics of MMP13-positive cells in tibia (C) . The statistics of MMP13-positive cells in meniscus (D) . The expression of Adamts5 and MMP13 in Western Blot (E) . The gray value statistics of Adamts5 (F) The gray value statistics of MMP13.
Article Snippet: HE staining solution, Toluidine blue staining powder and safranin O/fast green staining powder was buy from Sigma (United States), SDS-PAGE gel rapid preparation kit was attained from Beyotime Biotechnology (Shanghai)Co., Ltd. rapamycin was acquired from Shanghai yuanye Bio-Technology Co., Ltd., IL-1β was purchased from R&D Systems (Bio-Techne), 3-methyladenine (3-MA, autophagy inhibitor) was produced from TargetMol (United States),
Techniques: Immunofluorescence, Staining, Expressing, Western Blot